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Strain R1 (ATCC BAA-816) |
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Strain R1 (ATCC BAA-816) |
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Dr. Jane Tang, Ph.D. Collection Scientist Bacteriology Program ATCC 10801 University Blvd., Manassas, VA 20110 Tel: 703-365-2700 July 1, 2003 Re: Deinococcus radiodurans strain R1 (USUHS) Dear Dr. Tang, At the request of Dr. Owen White of The Institute for Genomic Research (TIGR, Rockville, MD), I am providing a frozen culture of the Deinococcus radiodurans strain (R1) that was subjected to whole genome sequencing and published in Science in 1999 [White et al. Science, 286, 1571-1577]. The strain sequenced by TIGR was provided by Kenneth W. Minton (formally of the Uniformed Services University (USUHS), Bethesda, MD). The USUHS D. radiodurans strain (originally named Micrococcus radiodurans) was acquired in 1986 by Kenneth W. Minton from Bevan E. B. Moseley of the Agricultural and Food Research Council, Institute of Food Research, Reading Laboratory, Shinfield, Reading, Berkshire RG2 9AT, UK. The strain provided to Dr. Minton by B. E. B. Mosely is cited by Moseley in J. Gen. Microbiol. (1972), 70, 399-409 as that originally isolated by Anderson et al. (1956). Thus, the strain currently maintained at USUHS has been the subject of a substantial number of D. radiodurans publications since its isolation in 1956, and was the one sequenced by TIGR. Please email me with any questions. The culture is being shipped on dry ice and contains a frozen culture in 50% glycerol. Sincerely,
Michael J. Daly CORRECTIONS AND CLARIFICATIONS Reports: "Genome
sequence of the radioresistant bacterium Deinococcus radiodurans R1"
by
O.White et al. (19 Nov. 1999, p. 1571). In this Report, it was
erroneously reported that the strain that was sequenced was the R1 type
strain of this species maintained at the ATCC under the designation 13939.
Recent work by a variety of groups performing sequencing experiments on the D.
radiodurans R1 type strain, ATCC 13939, shows that the D. radiodurans
strain used in the White et al. Report is slightly different.
Most differences appear to be single nucleotide substitutions, some of which
result in frameshift and amino acid sequence differences in the predicted
proteome for this strain versus the ATCC strain. The strain sequenced in
this Report by TIGR was provided by Kenneth W. Minton [formally of the
Uniformed Services University (USUHS), Bethesda, MD]. This strain was
originally named Micrococcus radiodurans and was provided to Minton
in 1986 by Bevan E. B. Moseley of the Agricultural and Food Research
Council, Institute of Food Research, Reading Laboratory, Shinfield, Reading,
Berkshire RG2 9AT, UK. The differences exhibited between the ATCC 13939
strain and the USUHS strain probably reflect mutations acquired during years
of separate culture in the Moseley and Minton laboratories.Thanks to Michael
J. Daly at USUHS, the sequenced D. radiodurans strain has been
registered with the ATCC and bears the designation BAA-816. The authors
sincerely apologize for any inconvenience this has caused the scientific
community. |
